Nordmark Biochemicals Products

Animal Free GMP Collagenase
GMP Collagenase
Research Collagenase
Related Products

Most commercially available collagenases are derived by fermentation of the natural collagenase producing bacteria species Clostridium histolyticum.

The strain of C. histolyticum, used in the manufacturing of our collagenase products, has been carefully selected for the production of large amounts of collagenases and balanced amounts of other proteolytic activities like neutral protease and clostripain.

As a pathogen, C. histolyticum uses these enzymatic activities to infect a potential host by digesting the extracellular matrix proteins of connective tissues. Therefore, the natural composition of these enzymes (collagenase class I and class II, neutral protease and clostripain) is already optimized by nature for most efficient tissue dissociation resulting in a high yield of viable cells.

Every application of collagenases places different demands on optimal digestion conditions. Nordmark  collagenase products include the range from standard collagenases, containing balanced amounts of other proteolytic activities, to highly purified collagenases in research or GMP grades, respectively. Therefore,our collagenase products are particularly suitable for various cell isolation applications.

Animal Free GMP Collagenase

Animal-Free (AF) products are our superior enzymes designed to provide the highest possible safety for clinical applications. A plant-based production process and the use of carefully selected materials ensure that there is no commingling with components of animal origin. Thus, any risk of introducing potential animal-derived pathogens is avoided in the first place. Furthermore, the sophisticated production process ensures that there is no cross-contamination with any animal-derived materials. The animal-free enzymes are produced in compliance with GMP guidelines that guarantees the highest quality and a reliable lot-to-lot consistency. Therefore, these enzymes provide the highest level of safety and reduced regulatory hurdles.

Collagenase AF-1 GMP Grade and Neutral Protease AF GMP Grade represent the first choice for clinical isolation of especially sensitive cells like pancreatic islets, hepatocytes and dental pulp.

Collagenase AF-1 GMP Grade
Neutral Protease AF GMP Grade


GMP Collagenase

Enzymes for tissue dissociation are critical raw materials for tissue engineering and transplantation into humans. Thus, Nordmark Biochemicals offers superior GMP grade Collagenase NB and Neutral Protease NB products that are manufactured in compliance with the EU-Guide to Good Manufacturing Practice (GMP). Furthermore, TSE safety of the products is certified and virus validation studies have been performed.

Superb quality and performance of our highest standard GMP Grade enzymes are assured by

  • stringent quality control
  • reliable lot-to-lot consistency
  • accurate analysis of proteolytic enzyme activities
  • in-process controls
  • manufacture according to GMP guidelines
Collagenase NB 1 GMP Grade
Collagenase NB 6 GMP Grade
Neutral Protease NB GMP Grade


Research Collagenase

Every application places different demands on optimal digestion conditions. Therefore, Nordmark offers several collagenase products with different enzymatic activities for research and protocol development in different application fields.

Nordmark collagenase products include the range from standard collagenases, containing balanced amounts of other proteolytic activities, to highly purified collagenases.

Collagenase NB products are particularly suitable for gentle and efficient dissociation of numerous tissue types leading to high yields of viable cells.

Collagenase NB 1 Premium Grade 
Collagenase NB 8 Broad Range
Collagenase NB 7D Gentle Grade
Collagenase NB 4G Proved Grade
Collagenase NB 5 Sterile Grade
Collagenase NB 4 Standard Grade
Neutral Protease NB
Neutral Protease NB High Active Grade
Clostripain NB


Related Products

Nordmark’s comprehensive portfolio for tissue digestion is completed by further products like Collagenase NB Reference Content, Collagenase NB Reference Identity, and Anti-Collagenase polyclonal antibodies.

The Collagenases N Reference products are available for enzyme activity determination or incoming goods inspection according to GMP.

Anti-Collagenase antibodies can be used for analysis of residual collagenase in cell preparations.

Collagenase N Reference Content
Collagenase N Reference Identity
Anti-Collagenase, polyclonal antibody from sheep
Anti-Collagenase HRP


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