Neutral Protease NB from Clostridium histolyticum

Nordmark Collagenase NB - first choice for high yield and viable cells

Metalloprotease suitable for tissue dissociation applications. For cell isolation Neutral Protease NB is used in combination with purified Collagenase NB 1 Premium Grade (Cat. no. S1745503) as well as Collagenase NB 4 Standard Grade (Cat. no. S1745401) or Collagenase NB 8 Broad Range (Cat. no. S1745601).
For research use only. If cells are intended for transplantation into humans, Neutral Protease NB GMP Grade (Cat. no. N0002936) is recommended.

Specifications:

Contains chromatographically purified neutral protease.
Neutral protease activity (DMC): ≥ 0.50 U/mg
Largely free of collagenolytic activity.
Mr ca. 34 000 • CAS [9001-92-7]

Order Information

Productname Cat.No Size EUR
Neutral Protease NB S3030111

50 DMC U

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Neutral Protease NB S3030112 100 DMC U Inquire+

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Storage Temperature: +2 °C to +8 °C
This article will be delivered cooled on blue ice.

Related Products

Neutral Protease NB is often used in combination with Collagenase NB 1 Premium Grade (Cat. No. S1745503). Neutral Protease NB can also be used with Collagenase NB 4 Standard Grade (Cat. No. S1745401) or Collagenase NB 8 Broad Range (Cat. No. S1745601), as well. If the application meets GMP requirements, Nordmark offers Neutral Protease NB GMP Grade (Cat. No. N0002936), which can be used in combination with Collagenase NB 1 GMP Grade (Cat. No. N0002937). Nordmark also offers animal-free products, Neutral Protease AF GMP Grade (Cat. No N0003553) and Collagenase AF-1 GMP Grade (Cat. No. N0003554) which are completely free of any animal based components excluding the risk of transmission of potential animal-derived pathogens.

References and Definitions

Unit definition: 1 DMC Unit catalyzes the cleavage of 1 µmole peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS (1).

References:
1. Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93.

Safety Information

Signal word

DANGER

Hazard Statements

H315 - H317 - H319 - H334 - H335

Precautions

P280

Reaction

P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 

EINECS: 232-582-9 • WGK: 1 • HS: 35079090

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