Neutral Protease NB High Active Grade from Clostridium histolyticum

Nordmark Collagenase NB - first choice for high yield and viable cells

Metalloprotease suitable for tissue dissociation applications. For cell isolation Neutral Protease NB High Active Grade is used in combination with highly purified Collagenase NB 1 Premium Grade (Cat. no. S1745503), Collagenase NB 4 Standard Grade (Cat. no. S1745401) or Collagenase NB 4G Proved Grade (Cat. no. S1746501).

Specifications:

Contains highly active, chromatographically purified neutral protease from Clostridium histolyticum.

Neutral protease activity (DMC): ≥ 4.00 U/mg.
Largely free of collagenolytic activity.
Endotoxin: ≤ 50 EU/mg (Ph. Eur.)
Mr ca. 34 000 • CAS [9001-92-7]

Order Information

Productname Cat.No Size EUR
Neutral Protease NB High Active Grade S3030202

80 DMC U

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Storage Temperature: +2 °C to +8 °C
This article will be delivered cooled on blue ice.

Related Products

Neutral Protease NB High Active Grade is used in combination with highly purified Collagenase NB 1 Premium Grade (Cat. No. S1745503), Collagenase NB 4 Standard Grade (Cat. No. S1745401) or Collagenase NB 4G Proved Grade (Cat. No. S1746502). If the application requires decreased proteolytic activity, Neutral Protease NB (Cat. No. S3030112) might be used. For applications under GMP requirements, Nordmark offers Neutral Protease NB GMP Grade (Cat. No. N0002936).

References and Definitions

Unit definition: 
Neutral Protease: 1 DMC Unit catalyzes the cleavage of 1 µmole peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS (1).
Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)). 

References:
1. Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93.

Safety Information

Signal word

DANGER

Hazard Statements

H315 - H317 - H319 - H334 - H335

Precautions

P280

Reaction

P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 

EG-Index: 647-014-00-9 • EINECS: 232-642-4 • WGK: 1 • HS: 35079090

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