Neutral Protease NB High Active Grade from Clostridium histolyticum
Nordmark Collagenase NB - first choice for high yield and viable cells
Metalloprotease suitable for tissue dissociation applications. For cell isolation Neutral Protease NB High Active Grade is used in combination with highly purified Collagenase NB 1 Premium Grade (Cat. no. S1745503), Collagenase NB 4 Standard Grade (Cat. no. S1745401) or Collagenase NB 4G Proved Grade (Cat. no. S1746501).
Contains highly active, chromatographically purified neutral protease from Clostridium histolyticum.
Neutral protease activity (DMC): ≥ 4.00 U/mg.
Largely free of collagenolytic activity.
Endotoxin: ≤ 50 EU/mg (Ph. Eur.)
Mr ca. 34 000 • CAS [9001-92-7]
|Neutral Protease NB High Active Grade||S3030202|| |
80 DMC U
+Please use the inquiry form in the right column.
Storage Temperature: +2 °C to +8 °C
This article will be delivered cooled on blue ice.
Neutral Protease NB High Active Grade is used in combination with highly purified Collagenase NB 1 Premium Grade (Cat. No. S1745503), Collagenase NB 4 Standard Grade (Cat. No. S1745401) or Collagenase NB 4G Proved Grade (Cat. No. S1746502). If the application requires decreased proteolytic activity, Neutral Protease NB (Cat. No. S3030112) might be used. For applications under GMP requirements, Nordmark offers Neutral Protease NB GMP Grade (Cat. No. N0002936).
References and Definitions
Neutral Protease: 1 DMC Unit catalyzes the cleavage of 1 µmole peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS (1).
Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).
1. Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93.
H315 - H317 - H319 - H334 - H335
P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362
EG-Index: 647-014-00-9 • EINECS: 232-642-4 • WGK: 1 • HS: 35079090