Collagenase NB 4G Provd Grade from Clostridium histolyticum

Nordmark Collagenase NB - first choice for high yield and viable cells

Suitable for isolation of a broad variety of cell types, especially for fibroblasts, human and rodent hepatocytes, frog oocytes and epithelial cells.

Specifications:

Contains class I and class II collagenase (1) and a balanced mix of neutral protease, clostripain and trypsin-like activities.
PZ activity (Wünsch): ≥ 0.18 U/mg
(Clostridiopeptidase A)
EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenase) • CAS [9001-12-1]

Order Information

Productname Cat.No Size EUR
Collagenase NB 4G Proved Grade S1746501

500 mg

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Collagenase NB 4G Proved Grade S1746502 1 g

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Collagenase NB 4G Proved Grade S1746503

5 mg

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Storage Temperature: +2 °C to +8 °C
 

Related Products

If you are dealing with poor cell viability, choose a product with less proteolytic activity like Collagenase NB 4 Standard Grade (Cat. Co. S1745401) or lower side activities like Collagenase NB 8 Broad Range (Cat. No. S1745602).

References and Definitions

Unit definition: 1 U according to Wünsch (2) catalyzes the hydrolysis
of 1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1. 

References:
1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler´s Z. Physiol. Chem. 333, 149-51

Safety Information

Signal word

DANGER

Hazard Statements

H315 - H317 - H319 - H334 - H335

Precautions

P280

Reaction

P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364

EINECS: 232-582-9 • WGK: 1 • HS: 35079090

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