Collagenase NB 1 GMP Grade from Clostridium histolyticum

Nordmark GMP enzymes - highest quality and safety for clinical applications

This highly purified collagenase quality is used for isolation of sensitive cells and guarantees high yields and viability. In combination with Neutral Protease NB GMP Grade (Cat. no. N0002936), Collagenase NB 1 GMP Grade is especially applicable to isolation of pancreatic islets of Langerhans.
Collagenase NB 1 GMP Grade is recommended for applications which have to meet GMP requirements like tissue engineering and transplantation into humans.

Specifications:

Contains chromatographically highly purified class I and class II collagenase (1).
Largely free from clostripain, trypsin-like protease and neutral protease.
Vial contains not less than 2000 PZ U collagenase.
PZ activity (Wünsch): ≥ 3.000 U/mg
Endotoxin ≤ 10.0 EU/mg (Ph. Eur.)
Microbiological examination: Total aerobic microbial count (TAMC), total combined yeast/moulds count (TYMC), absence of Clostridia, abnormal toxicity (general safety test; Ph. Eur.).
Manufactured according to EU GMP guidelines.
(Clostridiopeptidase A)
EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1]

Order Information

Productname Cat.No Size EUR
Collagenase NB 1 GMP Grade N0002937 ≥ 2,000 U* Inquire+

+Please use the inquiry form in the right column.

Storage Temperature: +2 °C to +8 °C
This article will be delivered cooled on blue ice.

Related Products

Collagenase NB 1 GMP Grade is often used in combination with Neutral Protease GMP Grade (Cat. No. N0002936). Nordmark also offers Collagenase AF-1 GMP Grade (Cat. No. N0003554) and Neutral Protease AF GMP Grade (Cat. No. N0003553) which are completely free of any animal based components excluding the risk of transmission of potential animal-derived pathogens. For research purpose, Nordmark also provides research grade alternatives like Collagenase NB 1 Premium Grade (Cat. No. S1745503) and Neutral Protease NB (Cat. No. S3030112).

References and Definitions

*Unit definitions: Collagenase: 1 U according to Wünsch (2) catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.
**Endotoxin: Ph. Eur. [1 Endotoxin Unit is equal to 1 International Unit of a WHO appoved reference standard endotoxin (RSE)].

References:
1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler´s Z. Physiol. Chem. 333, 149-51

Safety Information

Signal word

DANGER

Hazard Statements

H315 - H317 - H319 - H334 - H335

Precautions

P280

Reaction

P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364

EINECS: 232-582-9 • WGK: 1 • HS: 35079090

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