Application of Collagenase NB - By Organ & Tissue

Islets isolated with AF enzymes, Photo: Greg Szot, UCSF

Nordmark offers high quality Collagenase NB products which are particularly suitable for cell isolation from various tissues. Stringent production standards result in reliable lot-to-lot consistency and excellent performance. Collagenase NB is the first choice for researchers aiming for high yield of viable cells.

In this section application details are organized by organ and tissue applications which have been successfully isolated with Nordmark Collagenase NB products:

Adipose/Fat Bone Cartilage
Colon Gastric/Intestine Heart
Liver Lung Muscle
Neural Pancreas Reproductive
Skin Thyriod Tumor
Umbilical cord    

General remarks on Isolation Conditions 

In general,  the required Collagenase NB concentration depends on tissue type and origin as well as isolation procedure. Suggested concentrations should be considered as starting points and progress of the digestion process should be monitored visually in order to determine the optimal conditions.
We recommend dosing Collagenase NB by activity (PZ units), not by weight. Nordmark guarantees a high lot-to-lot consistency; however, small variations may occur. Therefore dosing in activity ensures consistent results for your cell isolation process. 
For further information on optimization of tissue digestion please visit our support section!

Isolation of cells intended for transplantation into humans

Enzymes for tissue digestion are critical raw materials for tissue engineering and transplantation into humans. Nordmark Biochemicals offers superior GMP Grade Collagenase NB products which are manufactured according to current GMP guidelines by the German pharmaceutical company Nordmark.  For further information please contact us!

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