Muscle Cells

Isolation of muscle cells with Collagenase NB

In the following, application details for isolation of cells from human skeletal muscle are presented.

Species Enzyme Conditions Download
Human* Collagenase NB 8 0.4-0.6 PZ U/ml,
  37 °C
Protocol

* For information on GMP grade Collagenase NB please contact us!

Collagenase NB 8 Broad Range (cat. no. S1745601) is characterized by a high  collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities. 

Isolation conditions
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results. 
For further information on optimization of tissue digestion please visit our support section!

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