Epithelial Cells

Isolation of epithelial cells with Collagenase NB

In the following, application details for isolation of epithelial cells from murine mammary glands, rodent gastric mucosa and human thyroid glands are presented.

 Species  Enzymes  Conditions  Download
 Human* Collagenase NB 4G 0.25-0.3 PZ U/ml, 
RT
Protocol
 Mouse Collagenase NB 4  0.3-0.4 PZ U/ml, 
15-25 min, 37 °C
Protocol
 Rodent Collagenase NB 4G 0.1-0.2 PZ U/ml, 
30-60 min, 37 °C
Protocol

 * For information on GMP grade Collagenase NB please contact us!

Collagenase NB 4 Standard Grade (cat. no. S1745402) has a collagenase activity of 
≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. 

Collagenase NB 4G Proved Grade (cat. no. S1746501) has a collagenase activity of 
≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.

Collagenase NB 6 GMP Grade (cat. no. N0002779)  has a collagenase activity of 
≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Isolation conditions
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results. For further information on optimization of tissue digestion please visit our support section!

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