Liver

Isolation of hepatocytes from liver tissue with Collagenase NB

In the following, application details for isolation of hepatocytes from rodent, porcine and human liver tissues are presented.

Species

 Enzymes

 Conditions

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Human*

 Collagenase NB 4G

0.25-0.35 PZ U/ml,
10-20 min, 37 °C

Protocol

 Mouse

 Collagenase NB 4G

0.08-0.11 PZ U/ml, 
5-15min, 37 °C

Protocol

 Pig

  Collagenase NB 8

0.2-0.3 PZ U/ml

Protocol

 Rat (adult)

 Collagenase NB 4G

 0.12-0.15 PZ U/ml, 
10-25 min, 37 °C

Protocol


Clinical applications*

Species      

Enzyme   

Conditions

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Human*

Collagenase NB 6

0.25-0.35 PZ U/ml,
10-20 min, 37 °C

Protocol


* For information on GMP grade Collagenase NB please contact us! 

Collagenase NB 4G Proved Grade (cat. no. S1746501) has a collagenase activity of 
≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.

Collagenase NB 6 GMP Grade (cat. no. N0002779)  has a collagenase activity of 
≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Collagenase NB 8 Broad Range (cat. no. S1745601)  is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities. 

Isolation conditions
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results. For further information on optimization of tissue digestion please visit our support section!

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