Collagenase NB – Significance and application
Collagenase, obtained from Clostridium histolyticum, is an enzyme used for tissue dissociation in vitro and thus for isolation of many different types of cells. These isolated cells are then used for numerous clinical and research applications.
Since 2002, Nordmark Arzneimittel GmbH & Co. KG has been producing various different grades of Collagenase NB, which were distributed via SERVA Electrophoresis GmbH, Heidelberg, Germany until the end of 2017. Since January 2018 Nordmark combines production, logistics and marketing on site. To meet the required high specifications, Nordmark produces Collagenase NB in plants specially constructed and qualified only for this purpose. All production steps take place in compliance with current international GMP guidelines.
Providing different quality levels of Collagenase NB ensures that the specific requirements related to cell isolation from different tissues and for different applications can be met. The use of a cell type-specific Collagenase NB grade ensures isolation of vital cells with a high yield.
Collagenase NB GMP grades– isolation of cells for sophisticated clinical applications
Our GMP compliant Collagenase NB qualities for the isolation of cells for use in a clinical setting meet the highest possible safety specifications.
Product safety is ensured by means of extensive microbiological controls. In addition, the products’ viral and TSE safety is assured by means of careful selection of the starting materials and the specific production process.
At present, Collagenase NB has found highly promising applications in regenerative medicine and tissue engineering. Human cells are isolated and grown, if applicable, into tissue in the laboratory. The cells or tissue are suitable for human transplantation either for regeneration purposes or to replace damaged tissue.
Some examples of cell types used in the clinical setting:
- Pancreatic islets of Langerhans
- Cartilage cells
- Liver cells
- Skin cells (e.g. fibroblasts)
- Stem cells from adipose, placental or umbilical cord tissue
Collagenases – valuable tools for research
In preparation for the clinical use of these cells, a substantial research phase is necessary in order to obtain information about the growth or possible rejection of the tissue. Moreover, our Collagenase NB grades are valuable tools in many other areas of research.
Collagenase NB is used for the isolation of many different types of cells, for example, from tumours, the lungs or the intestine, with the goal of examining the metabolism, environmental reactions or growth of these cells. The information obtained can help researchers better understand the processes taking place in the cells and therefore in a living organism. This information is also useful in the development of new drugs or therapies.