Collagenase NB 8 Broad Range-The Extra Push
For Cell Isolation of Rodent Pancreas and Tumors
Collagenase NB 8 Broad Range is a chromatographically purified enzyme resulting in a high collagenolytic activity. This product has an increased collagenase activity compared to Collagenase NB 4 Standard Grade, Collagenase NB 4G Proved Grade and Collagenase NB 7D Gentle Grade. The additional enzymatic activities like clostripain, trypsin-like activity and neutral protease, as well as the endotoxin level are reduced. Collagenase NB 8 is suitable for a broad range of different applications in tissue dissociation such as isolation of rodent pancreatic islets of Langerhans, human cardiomyocytes, tumor cells, muscle cells, osteoblasts and porcine hepatocytes.
Product Information
| Origin | Clostridium histolyticum |
| Composition | Contains class I and class II collagenase1 as well as reduced levels of clostripain, trypsin-like activities and neutral protease Chromatographically purified |
| Molecular weight | 70 – 120 kDa (collagenases) |
| Special features | - |
| Microbiological examination | Endotoxin*: ≤ 100 EU/mg |
| Activities | Collagenase activity: ≥ 0.90 U/mg (PZ activity** acc. to Wünsch, 25 °C) |
| Storage conditions | +2 °C to +8 °C |
| Shipping conditions | At ambient temperature worldwide |
| Intended use | For research use only |
Unit definition:
*Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).
**PZ Unit (acc. to Wünsch2):
1 U according to Wünsch catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.
References:
- Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
- Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51
Related Products
If you are dealing with poor cell yields, try collagenases with higher side activities like Collagenase NB 4 Standard Grade and Collagenase NB 4G Proved Grade.
Ordering Information
| Product Name | Cat. No. | Size | Contact us | Order |
|---|---|---|---|---|
| Collagenase NB 8 Broad Range | S1745601 | 250 mg | Inquire | Webshoplink |
| Collagenase NB 8 Broad Range | S1745602 | 1 g | Inquire | Webshoplink |
Documentation
SDS NB 8 Broad Range122 KB
CoO NB 8 Broad Range890 KB
Find your Certificate of Analysis here!
Safety Information
| Signal word | DANGER |
| Hazard statements | H315 - H317 - H319 - H334 - H335 |
| Precautions | P280 |
| Reaction | P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364 |
EINECS: 232-582-9 • WGK: 1 • HS: 35079090 • EC 3.4.24.3 • CAS [9001-12-1]
