Collagenase NB 4G Proved Grade-Extra Side Activities
For Isolation of Hepatocytes, Fibroblasts and Epithelial Cells
Collagenase NB 4G Proved Grade is an enzyme mixture which contains collagenolytic and additional enzymatic activities including clostripain and neutral protease. The ratio of these activities leads to gentle and efficient tissue dissociation. The product is suitable for isolation of a broad variety of cell types, especially for fibroblasts, human and rodent hepatocytes, frog oocytes and epithelial cells.
Product Information
| Origin | Clostridium histolyticum |
| Composition | Contains class I and class II collagenase1 and a balanced mix of neutral protease, clostripain and trypsin-like activities |
| Molecular weight | 70 – 120 kDa (collagenases) |
| Special features | - |
| Microbiological examination | - |
| Activities | Collagenase activity: ≥ 0.18 U/mg (PZ activity* acc. to Wünsch, 25 °C) |
| Storage conditions | +2 °C to +8 °C |
| Shipping conditions | At ambient temperature worldwide |
| Intended use | For research use only |
*PZ Unit (acc. to Wünsch2):
1 U according to Wünsch catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.
References:
- Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
- Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51
Related Products
If you are dealing with poor cell viability, choose a product with less proteolytic activity like Collagenase NB 4 Standard Grade or lower side activities like Collagenase NB 7D Gentle Grade or Collagenase NB 8 Broad Range.
Ordering Information
| Product Name | Cat. No. | Size | Contact us | Order |
|---|---|---|---|---|
| Collagenase NB 4G Proved Grade | S1746501 | 500 mg | Inquire | Webshoplink |
| Collagenase NB 4G Proved Grade | S1746502 | 1 g | Inquire | Webshoplink |
| Collagenase NB 4G Proved Grade | S1746503 | 5 g | Inquire | Webshoplink |
Documentation
Product Info NB 4G52 KB
SDS NB 4G Proved Grade122 KB
CoO NB 4G Proved Grade894 KB
Find your Certificate of Analysis here!
Safety Information
| Signal word | DANGER |
| Hazard statements | H315 - H317 - H319 - H334 - H335 |
| Precautions | P280 |
| Reaction | P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364 |
EINECS: 232-582-9 • WGK: 1 • HS: 35079090 • EC 3.4.24.3 • CAS [9001-12-1]
