Collagenase NB 1 Premium Grade-With High Activity
For Cell Isolation from Pancreas, other Organs and Tissues
Collagenase NB 1 Premium Grade is a highly purified collagenase grade used for isolation of sensitive cells and guarantees high yields and viability. In combination with Neutral Protease NB Collagenase NB 1 Premium Grade is especially applicable for isolation of pancreatic islets of Langerhans from different species like human or pig. For research use only. If cells are intended for transplantation into humans, Collagenase NB 1 GMP Grade is recommended.
Product Information
| Origin | Clostridium histolyticum |
| Composition | Contains chromatographically highly purified class I and class II collagenase1 Largely free from clostripain, trypsin-like protease and neutral protease |
| Molecular weight | 70 – 120 kDa (collagenases) |
| Special features | - |
| Microbiological examination | Endotoxin*: ≤ 10.0 EU/mg |
| Activities | Collagenase activity: ≥ 3.00 U/mg lyophilisate (PZ activity** acc. to Wünsch, 25 °C) |
| Storage conditions | +2 °C to +8 °C |
| Shipping conditions | At ambient temperature worldwide |
| Intended use | For research use only |
Unit definition:
*Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).
**PZ Unit (acc. to Wünsch2):
1 U according to Wünsch catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.
References:
- Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
- Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51
Related Products
Collagenase NB 1 Premium Grade is often used in combination with Neutral Protease NB. If the application demands GMP requirements, Nordmark offers Collagenase NB 1 GMP Grade that can be used in combination with Neutral Protease NB GMP Grade. Nordmark also offers animal-free products, Collagenase AF-1 GMP Grade and Neutral Protease AF GMP Grade, which are completely free of any animal-based components, thereby excluding the risk of transmission of potential animal-derived pathogens.
Ordering Information
| Product Name | Cat. No. | Size | Contact us | Order |
|---|---|---|---|---|
| Collagenase NB 1 Premium Grade | S1745503 | ≥ 2000 PZ U* | Inquire | Webshoplink |
Documentation
Product Info NB 154 KB
SDS NB 1 Premium Grade108 KB
CoO NB 1 Premium Grade881 KB
Find your Certificate of Analysis here!
Safety Information
| Signal word | DANGER |
| Hazard statements | H315 - H317 - H319 - H334 - H335 |
| Precautions | P280 |
| Reaction | P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364 |
EINECS: 232-582-9 • WGK: 1 • HS: 35079090 • EC 3.4.24.3 • CAS [9001-12-1]
