Isolation of Neurons with Collagenase NB
Protocol Recommendations
In the following, application details for isolation of neurons from human and rodent dorsal root ganglia and Schwann cells from nerve tissue are presented.
Neural
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Research | Collagenase NB 4 Standard Grade | 0.15 - 0.25 PZ U/ml, 30 - 60 min, 37 °C | Details |
| Human | Clinic | Collagenase NB 6 GMP Grade | 0.15 - 0.25 PZ U/ml, 30 - 60 min, 37 °C | Details |
| Rodent | Research | Collagenase NB 4 Standard Grade | 0.15 - 0.25 PZ U/ml, 30-60 h, 37 °C | Details |
Schwann Cells from Neuronal Tissue
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Clinic | Collagenase NB 1 GMP Grade & Neutral Protease NB GMP Grade | 0.5 PZ U/ml, 2 DCM U/ml, 18 h, 37 °C | Details |
| Rodent | Research | Collagenase NB 4 Standard Grade | 0.2 - 0.3 PZ U/ml, 1-3 h, 37 °C | Details |
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Brief Product Information
Collagenase NB 6 GMP Grade
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
Collagenase NB 1 GMP Grade
This collagenase is a highly purified enzyme with a high collagenolytic activity (≥ 3.0 PZ U/mg) and very low proteolytic side activities and is also available as the research grade Collagenase NB 1 Premium Grade is also available.
Neutral Protease NB GMP Grade
This neutral protease is a protease from C. histolyticum. It has a proteolytic activity of ≥ 0.50 DMC U/mg and is also available as the research grade Neutral Protease NB.
Collagenase NB 4 Standard Grade
This product has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
Isolation Note
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
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