Isolation of Heart Cells with Collagenase NB
Protocol Recommendations
In the following, application details for isolation of cardiomyocytes from rodent and human heart tissue and endothelial cells from rodent aortas are presented.
Cardiomyocytes from Cardiac Muscle Tissue:
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Rat (adult) | Research | Collagenase NB 8 Broad Range | 0.5 PZ U/ml, 45 - 60 min, 37 °C | Details |
| Human | Research+ | Collagenase NB 8 Broad Range | 0.8 - 1.0 PZ U/ml, 2 - 3.5 h, 37 °C | Details |
Endothelia Cells from Aorta Tissue:
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Rodent | Research | Collagenase NB 4 Standard Grade | 0.12 - 0.15 PZ U/ml, 10 - 20 min, 37 °C | Details |
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Brief Product Information
Collagenase NB 8 Broad Range
This product is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
Collagenase NB 4 Standard Grade
This product has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
Isolation Note
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
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