Isolation of Chondrocytes from Cartilage with Collagenase NB
Protocol Recommendations
In the following, application details for isolation of chondrocytes from murine, bovine and human cartilage are presented.
Cartilage
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Mouse (newborn) | Research | Collagenase NB 4 Standard Grade & Neutral Protease NB | Consecutive digestion steps: 0.5 - 1 DMC U/ml(NP), 15 - 20 min, 37 °C 0.3 PZ U/ml (NB 4), 30 - 45 min, 37 °C | Details & Publication |
| Bovine | Research | Collagenase NB 4 Standard Grade | 0.2 - 0.3 PZ U/ml, 14 - 16 h, 37 °C | Details |
| Human | Research | Collagenase NB 4 Standard Grade | 0.3 - 0.4 PZ U/ml, 4 - 16 h, 37 °C | Details |
| Human | Clinic | Collagenase NB 6 GMP Grade | 0.3 - 0.4 PZ U/ml, 4 - 16 h, 37 °C | Details |
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Brief Product Information
Collagenase NB 6 GMP Grade
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
Collagenase NB 4 Standard Grade
This product has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
Neutral Protease NB
This product is a protease from C. histolyticum. It has a proteolytic activity of ≥ 0.50 DMC U/mg.
Isolation Note
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
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