Isolation of Adipocytes, ADSCs and SVF with Collagenase NB
Protocol Recommendations
In the following, application details for isolation of adipocytes from human or murine adipose tissue are presented.
Adipocytes & Adipose-Derived Stem Cells
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Murine | Research | Collagenase NB 4 Standard Grade | 0.2 - 0.3 PZ U/ml*, 30 - 60 min, 37 °C | Details |
| Human | Research | Collagenase NB 4 Standard Grade | 0.2 - 0.3 PZ U/ml*, 30 - 60 min, 37 °C | Details |
| Human | Clinic | Collagenase NB 6 GMP Grade | 0.2 - 0.3 PZ U/ml*, 30 - 60 min, 37 °C | Details |
Stromal Vascular Fraction (Stem Cells)
| Species | Application Field | Enzymes | Experimental Conditions | Downloads |
|---|---|---|---|---|
| Human | Clinic | Collagenase AF-1 GMP Grade & Neutral Protease AF GMP Grade | 0.4 PZ U/ml & 0.2 DMC U/ml**, 50 min, 37 °C | Details & Laboratory Note |
| Human | Clinic | Collagenase NB 6 GMP Grade | 0.12 - 0.25 PZ U/ml, 45 - 60 min, 37 °C | Publication 1 & Publication 2 |
* 0.1 - 0.15 PZ U/ml final concentration after addition of adipose tissue
** 0.2 PZ U/ml & 0.1 DMC U/ml final concentration after addition of adipose tissue
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Brief Product Information
Collagenase NB 6 GMP Grade
This collagenase has an activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
Collagenase AF-1 GMP Grade
This collagenase is a highly purified enzyme with a high collagenase activity of ≥ 3.00 U/mg. It is produced under GMP guidelines and completely free of any animal-based components.
Neutral Protease AF GMP Grade
This neutral protease is a highly purified enzyme with a proteolytic activity of ≥ 0.50 DMC U/mg. It is produced under GMP guidelines and completely free of any animal-based components.
Collagenase NB 4 Standard Grade
This product has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
Isolation Note
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units and DMC units for Neutral Protease NB to ensure consistent results.
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