Collagenase NB 8 Broad Range from Clostridium histolyticum

Nordmark Collagenase NB - first choice for high yield and viable cells

Suitable for a broad range of different applications in tissue dissociation such as isolation of rodent pancreatic islets of Langerhans, human cardiomyocytes, tumor cells, muscle cells, osteoblasts and porcine hepatocytes. Increased collagenase activity compared to Collagenase NB 4 Standard Grade (Cat. no. S1745401) and Collagenase NB 4G Proved Grade (Cat. no. S1746501).

Specifications:

Contains class I and class II collagenase (1) as well as reduced levels of clostripain, trypsin-like activities and neutral protease.
Chromatographically purified.
PZ activity (Wünsch): ≥ 0.90 U/mg
Endotoxin: ≤ 100 EU/mg (Ph. Eur.)
(Clostridiopeptidase A)
EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1]

Order Information

Productname Cat.No Size EUR
Collagenase NB 8 Broad Range S1745601

250 mg

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Collagenase NB 8 Broad Range

S1745602 1 g

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Storage Temperature: +2 °C to +8 °C
This article will be delivered cooled on blue ice.

Related Products

If you are dealing with poor cell yields, collagenases with higher side activities like Collagenase NB 4 Standard Grade (Cat. No. S1745401) and Collagenase NB 4G Proved Grade (Cat. Co. S1746502).

References and Definitions

Unit definition: 
Collagenase: 1 U according to Wünsch (2) catalyzes the hydrolysis of
1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.
Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).

References:
1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51.

Safety Information

Signal word

DANGER

Hazard Statements

H315 - H317 - H319 - H334 - H335

Precautions

P280

Reaction

P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 

EINECS: 232-582-9 • WGK: 1 • HS: 35079090

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