Nordmark Collagenase NB - first choice for high yield and viable cells
Collagenase NB 7D Gentle Grade is suitable for isolation of a broad variety of cells. Moreover, it can be used especially where sensitive conditions are favored as during isolation of dendritic and tumor cells from human or animal tissues. To meet these requirements, Collagenase NB 7D Gentle Grade combines the benefits of slightly higher collagenase activity with low tryptic activities ideal to preserve cell-surface proteins.
Collagenase NB 7D Gentle Grade can be used for a variety of different applications when sensitive cells are isolated.
Contains higher amounts of class I and class II collagenase (1) and lower trypsin-like activities.
PZ activity (Wünsch): ≥ 0.2 U/mg
Trypsin-like activities BAEE): ≤ 0.2 U/mg
Ratio Collagenase : Trypsin: ≥ 3.0
EC 188.8.131.52 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1]
|Collagenase NB 7D Gentle Grade||S1747401|
|Collagenase NB 7D Gentle Grade||S1747402||1 g|
|Collagenase NB 7D Gentle Grade||S1747403||5 g||Inquire+|
+Please use the inquiry form in the right column.
Storage Temperature: +2 °C to +8 °C
If you are dealing with less efficient cell isolation results, a product with higher tryptic activities like, Collagenase NB 4G Proved Grade (Cat. No. S1746502) might be better suitable. However, in case of poor cell viability, try a product with even less tryptic activities like Collagenase NB 1 Premium Grade (Cat. No. S1745503).
Collagenase: 1 U according to Wünsch (2) catalyzes the hydrolysis of
1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.
Trypsin-like activities (BAEE assay): 1 U catalyzes the cleavage of 1 µmol BAEE per minute at 25 °C, pH 8.0. Enzyme dissolved in 1 mM HCl.
1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51.
H315 - H317 - H319 - H334 - H335
P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362
EINECS: 232-582-9 • WGK: 1 • HS: 35079090