Collagenase NB 4 Standard Grade from Clostridium histolyticum

Nordmark Collagenase NB - first choice for high yield and viable cells

As Collagenase NB 4 Standard Grade has comparable enzymatic properties to Collagenase NB 5 Sterile Grade (Cat. no. N0002778) and Collagenase NB 6 GMP Grade (Cat. no.N0002779), this enzyme might also be used in order to establish applications which have to meet sterile or GMP requirements like tissue engineering and transplantation.

Specifications:

Contains class I and class II collagenase (1) and a balanced ratio of proteolytic side activities.
PZ activity (Wünsch): ≥ 0.10 U/mg
Suitable for different applications in tissue dissociation such as isolation of human and rodent hepatocytes, chondrocytes, neuronal and endothelial cells, fibroblasts, cells from adipose tissue (e.g. ADSCs), stem cells and rodent osteoblasts.
(Clostridiopeptidase A)
EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1]

Order Information

Productname Cat.No Size EUR

Collagenase NB 4 Standard Grade

S1745402 500 mg

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Collagenase NB 4 Standard Grade S1745401

1 g

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Collagenase NB 4 Standard Grade

S1745403 5 g

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Storage Temperature: +2 °C to +8 °C
 

Related Products

Collagenase NB 4 Standard Grade is often used to establish applications and to optimize protocols. If later on, the application meets sterile or GMP requirements, Collagenase NB 5 Sterile Grade (Cat. No. N0002778) or Collagenase NB 6 GMP Grade (Cat. No. N0002779) can be used. Both products have comparable enzymatic properties to Collagenase NB 4 Standard Grade.

References and Definitions

Unit definition: 1 U according to Wünsch (2) catalyzes the hydrolysis of
1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.

References:
1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51

Safety Information

Signal word

DANGER

Hazard Statements

H315 - H317 - H319 - H334 - H335

Precautions

P280

Reaction

P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 - +P364

EINECS: 232-582-9 • WGK: 1 • HS: 35079090

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