Collagenase NB 1 Premium Grade from Clostridium histolyticum

Nordmark Collagenase NB - first choice for high yield and viable cells

This highly purified collagenase grade is used for isolation of sensitive cells and guarantees high yields and viability. In combination with Neutral Protease NB (Cat. no. S3030112) Collagenase NB 1 Premium Grade is especially applicable to isolation of pancreatic islets of Langerhans from different species like human or pig.
For research use only. If cells are intended for transplantation into humans,
Collagenase NB 1 GMP Grade (Cat. no. N0002937) is recommended.

Specifications:

Contains chromatographically highly purified class I and class II collagenase (1).
Largely free from clostripain, trypsin-like protease and neutral protease.
Vial contains not less than 2000 PZ U collagenase.
PZ activity (Wünsch): ≥ 3.00 U/mg
Endotoxin: ≤ 10.0 EU/mg (Ph. Eur.)
(Clostridiopeptidase A)
EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1]

Order Information

Productname Cat.No Size EUR
Collagenase NB 1 Premium Grade S1745503

≥ 2000 PZ U

Inquire+

+Please use the inquiry form in the right column.

Storage Temperature: +2 °C to +8 °C
This article will be delivered cooled on blue ice.

Related Products

Collagenase NB 1 Premium Grade is often used in combination with Neutral Protease NB (Cat. No. S3030112). If the application meets GMP requirements, Nordmark offers Collagenase NB 1 GMP Grade (Cat. No. N0002937) that can be used in combination with Neutral Protease NB GMP Grade (Cat. No. N0002936). Nordmark also offers animal-free products, Collagenase AF-1 GMP Grade (Cat. No. N0003554) and Neutral Protease AF GMP Grade (Cat. No N0003553) which are completely free of any animal based components excluding the risk of transmission of potential animal-derived pathogens.

References and Definitions

Unit definition: 
Collagenase: 1 U according to Wünsch (2) catalyzes the hydrolysis of
1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.
Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).

References:
1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51.

Safety Information

Signal word

DANGER

Hazard Statements

H315 - H317 - H319 - H334 - H335

Precautions

P280

Reaction

P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 

EINECS: 232-582-9 • WGK: 1 • HS: 35079090

Product Inquiry

product inquire

product inquire sidebar

Personal Consulting

E-Mail

 

Phone:
+49 (0)4122 / 712 560