Clostripain NB from Clostridium histolyticum

Nordmark Clostripain NB - Fine tune your cell isolation

Clostripain also known as Clostridiopeptidase B or Endoproteinase Arg-C is a proteinase that cleaves peptide bonds on the carboxyl side of arginine. This protein is a heterodimer composed of a heavy and a light chain that are held together via strong noncovalent forces. As a proteolytic enzyme it can be used for isolation of various cell types by tissue dissociation.

Clostripain exists in two different forms, an oxidized, native condition and a reduced activated form that differ in enzymatic activity. The native clostripain can be activated with reducing agents (e.g. DTT, Cysteine) resulting in a higher activity of the enzyme.

Clostripain NB is used in combination with purified Collagenase NB 1 Premium Grade (Cat. no. S1745503) and/or Neutral Protease NB (Cat. No. S3030112) as well as Collagenase NB 4 Standard Grade (Cat. no. S1745401) or Collagenase NB 8 Broad Range (Cat. no. S1745601).
For research use only.

Specifications:

Contains chromatographically highly purified clostripain.
Clostripain, native state activity: ≥ 5.0 U/mg (BAEE)
Clostripain, activated activity: ≥ 50.0 U/mg (BAEE)
Clostripain activity, native state, per vial: ≥ 200 U/vial (BAEE)
Clostripain activity, activated, per vial: status
EC 3.4.24.3 • Mr ca. 59 000 • CAS [9028-00-6]

Order Information

Productname Cat.No Size EUR
Clostripain NB S2020501 ≥ 200 U Inquire+

+Please use the inquiry form in the right column.

Storage Temperature: -15 °C to -25 °C
This article will be delivered on dry ice.

References and Definitions

Unit definition:

Clostripain, native state (BAEE assay): 1 Unit catalyzes the cleavage of 1 µmol Nα-Benzoyl-L-arginine ethyl ester per minute at 25 °C, pH 7.8.

Clostripain, activated (BAEE assay): 1 Unit catalyzes the cleavage of 1 µmol Nα-Benzoyl-L-arginine ethyl ester per minute at 25 °C, pH 7.8. The enzyme is activated with 2.5 mM DTT.

References

1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091

2. Kula et al. (1976) Biochem. Biophys. Res. Commun. 69, 389-396

Safety Information

Signal word

DANGER

Hazard Statements

H315 - H317 - H319 - H334 - H335

Precautions

P280

Reaction

P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362+ P64

EINECS: 232-822-2 • WGK: 1 • HS: 35079090

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