Neutral Protease NB GMP Grade from Clostridium histolyticum

Nordmark GMP enzymes - highest quality and safety for clinical applications

Metalloprotease suitable for tissue dissociation applications. For cell isolation Neutral Protease NB GMP Grade is used in combination with purified Collagenase NB 1 GMP Grade (Cat. no. N0002937) or Collagenase NB 6 GMP Grade (Cat. no. N0002779) for applications which have to meet GMP requirements like tissue engineering and transplantation into humans.

Specifications:

Contains chromatographically purified neutral protease.
Vial contains not less than 100 DMC U of neutral protease.
Neutral protease activity (DMC): ≥ 0.50 U/mg
Endotoxin: ≤ 100 EU/mg (Ph. Eur.)
Largely free of collagenolytic activity.
Microbiological examination: Total aerobic microbial count (TAMC), total combined yeast/moulds count (TYMC), absence of Clostridia, abnormal toxicity (general safety test; Ph. Eur.).
Manufactured according to EU GMP guidelines.
Mr ca. 34 000 • CAS [9001-92-7]

Order Information

Productname Cat.No Size EUR
Neutral Protease NB GMP Grade  N0002936

≥ 100 DMC U

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Storage Temperature: +2 °C to +8 °C
This article will be delivered cooled on blue ice.

Related Products

For cell isolation, Neutral Protease NB GMP Grade is often used in combination with Collagenase NB 1 GMP Grade (Cat. No. N0002937). Nordmark also offers animal-free products, Neutral Protease AF GMP Grade (Cat. No N0003553) and Collagenase AF-1 GMP Grade (Cat. No. N0003554) which are completely free of any animal based components excluding the risk of transmission of potential animal-derived pathogens. For research purpose, Nordmark provides research grade alternatives like Neutral Protease NB (Cat. No. S3030112) and Collagenase NB 1 Premium Grade (Cat. No. S1745503).

References and Definitions

Unit definition: 
Neutral Protease: 1 DMC Unit catalyzes the cleavage of 1 µmole peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS (1).
Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).

References:
1. Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93.

Safety Information

Signal word

DANGER

Hazard Statements

H315 - H317 - H319 - H334 - H335

Precautions

P280

Reaction

P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364

EINECS: 232-582-9 • WGK: 1 • HS: 35079090

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