Collagenase AF-1 GMP Grade from Clostridium histolyticum

World's first animal free GMP Grade Collagenase

Collagenase AF-1 GMP Grade is derived from C. histolyticum using a production process completely free of any animal based components. Thus, any risk of transmission of potential animal-derived pathogens is excluded. This highly purified collagenase, often in combination with Neutral Protease AF GMP Grade (Cat. No. N0002936), is suitable for the dissociation of sensitive cells from several tissues, such as pancreas, liver and dental pulp. It has been shown to be highly effective for the isolation of islets of Langerhans from human pancreas intended for transplantation.

Application:

Collagenase AF-1 GMP can be used for several cell isolations, but is commonly used for human islet isolation. More about Applications

Specifications:

PZ activity (Wünsch) ≥ 3.00 U/mg*.

Endotoxin ≤ 10.0 EU**/mg (Ph. Eur.)

Microbiological examination: Total aerobic microbial count (TAMC), total combined yeast/moulds count (TYMC), absence of Clostridia.

Endproduct is 0.22 µm filtered.

Manufactured according to EU GMP guidelines.

(Clostridiopeptidase A, Collagenase A)
EC 3.4.24.3 • Mr Mr 70 000 - 120 000 (collagenase) • CAS [9001-12-1]

Order Information

Productname Cat.No Size EUR
Collagenase AF-1 GMP Grade N0003554 ≥ 2,000 U* Inquire+

+Please use the inquiry form in the right column.

Storage Temperature: +2 °C to +8 °C
This article will be delivered cooled on blue ice.

Related Products

Collagenase AF-1 GMP Grade is often used in combination with Neutral Protease AF GMP Grade (Cat. No. N0003553). For research purpose, Nordmark provides research grade alternatives like Collagenase NB 1 Premium Grade (Cat. No.  S1745503) and Neutral Protease NB (Cat. No. S3030112).

References and Definitions

*Unit definitions: Collagenase: 1 U according to Wünsch (2) catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.
**Endotoxin: Ph. Eur. [1 Endotoxin Unit is equal to 1 International Unit of a WHO appoved reference standard endotoxin (RSE)].

References:
1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler´s Z. Physiol. Chem. 333, 149-51

Safety Information

Signal word

DANGER

Hazard Statements

H315 - H317 - H319 - H334 - H335

Precautions

P280

Reaction

P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364

 

Product Inquiry

product inquire

product inquire sidebar

Personal Consulting

E-Mail

 

Phone:
+49 (0)4122 / 712 560