Umbilical cord

Isolation of cells from the umbilical cord with Collagenase NB

In the following, application details for isolation of human foreskin (HDMEC) and stem cells from human Wharton’s jelly and human or murine adipose tissue are presented

Endothelia cells from umbilical vein:

Species

 Enzymes

 Conditions

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 Human*
 (HUVEC)

Collagenase NB 4
or
Collagenase NB 6

0.15-0.25 PZ U/ml, 
0.5-2 h, 37 °C

Protocol


Stem cells from human Wharton's jelly:

Species

Enzyme

Conditions

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Human

Collagenase NB 4

0.13-0.15 PZ U/ml,
1-6 h , 37 °C

Protocol


Clinical applications*

Species

Enzyme

Conditions

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Human

Collagenase NB 6

0.13-0.15 PZ U/ml,
1-6 h , 37 °C

Protocol


* For information on GMP grade Collagenase NB please contact us!

Collagenase NB 4 Standard Grade (cat. no. S1745402) has a collagenase activity of 
≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. 

Collagenase NB 6 GMP Grade (cat. no. N0002779)  has a collagenase activity of 
≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Isolation conditions
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results. For further information on optimization of tissue digestion please visit our support section!

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