Pancreas

Isolation of pancreatic cells with Collagenase NB

In the following, application details for human Acinar cells, isolation of islets of Langerhans from human, rodent and porcine pancreatic tissue are presented.

Acinar cells from pancreatic tissue:

Species

Enzyme

 Conditions

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 Human*

 Collagenase NB 8

0.05-0.1 PZ U/ml, 
1 h, 37 °C

Protocol


Islets of Langerhans from pancreatic tissue:

Species

Enzyme

 Conditions

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Human*

Collagenase NB 1
Premium Grade,
Neutral Protease NB

Per gram tissue:
20 PZ U (NB 1),
0.8-1.5 DMC U (NP),
15-30 min, 37 °C

 

Porcine
(adult)

Collagenase NB 8,
Neutral Protease NB

Per gram tissue:
4.4 PZ U (NB 8),
0.5-0.7 DMC U (NP),
37 °C

Protocol

Porcine
(neonatal)

Collagenase NB 4

0.17-0.2 PZ U/ml,
10-20 min, 37 °C

Protocol

Rodent

Collagenase NB 8

1.5-1.7 PZ U/ml
14-17 min, 37 °C

Protocol

Human*

Collagenase NB 1
Premium Grade,
Neutral Protease NB

Per gram tissue:
20 PZ U (NB 1),
0.8-1.5 DMC U (NP),
15-30 min, 37 °C

 

 
Clinical Applications*

Species

Enzymes

Conditions

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Human

Collagenase NB 1
GMP Grade,
Neutral Protease NB
GMP Grade

Per gram tissue:
20 PZ U (NB 1),
0.8-1.5 DMC U (NP),
15-30 min, 37 °C

 

Porcine
(adult)

Collagenase NB 1
GMP Grade,
Neutral Protease NB
GMP Grade

Per gram tissue:
15-18  PZ U (NB 1),
0.8-1.3 DMC U (NP),
15-30 min, 37 °C

 

Porcine
(neonatal)

Collagenase NB 6

0.17-0.2 PZ U/ml,
10-20 min, 37 °C

 


* For information on GMP grade Collagenase NB please contact us!

Collagenase NB 1 Premium Grade (cat. no. S1745504)  s a highly purified collagenase with a high collagenolytic activity (≥ 3.0 PZ U/mg) and very low proteolytic side activities. It is also available as GMP conformant Collagenase NB 1 GMP Grade (cat. no. N0002937).

Neutral Protease NB (cat. no. S3030111) is a protease from C. histolyticum. It has a proteolytic activity of ≥ 0.50 DMC U/mg. It is also available as GMP conformant Neutral Protease NB GMP Grade (cat. no. N0002936). 

Collagenase NB 4 Standard Grade (cat. no. SD1745401)  has a collagenase activity of 
≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.

Collagenase NB 6 GMP Grade (cat. no. N0002779) has a collagenase activity of 
≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Collagenase NB 8 Broad Range (cat. no. S1745601) is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities. 

Isolation conditions
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results. 
For further information on optimization of tissue digestion please visit our support section!

Contact Nordmark Biochemicals Team

 

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