Lung

Isolation of lung cells with Collagenase NB

In the following, application details for isolation of cells from murine lung and human lung tumor cells are presented

Species

Enzyme

Conditions

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 Mouse

Collagenase NB 4

 0.2 PZ U/ml,
1 h, 37 °C

Protocol

Human*

Collagenase NB 8

3.5-5.5 PZ U/ml,
3-5 h, 37 °C

Protocol


* For information on GMP grade Collagenase NB please contact us! 

Collagenase NB 4 Standard Grade (cat. no. S1745401) has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.

Collagenase NB 8 Broad Range (cat. no. S1745601)  is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities. 

Isolation conditions
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results. 
For further information on optimization of tissue digestion please visit our support section!

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