Cartilage

Isolation of chondrocytes with Collagenase NB

In the following, application details for isolation of chondrocytes from murine, bovine and human cartilage are presented.

Species

 Enzyme

 Conditions

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 Bovine

Collagenase NB 4

0.2-0.3 PZ U/ml, 
14-16 h, 37 °C

Protocol

 Human*

Collagenase NB 4

0.3-0.4 PZ U/ml, 
4-16 h, 37 °C

Protocol

 Mouse
(newborn)

Neutral Protease NB,
Collagenase NB 4

Consecutive
digestion steps:
0.5-1 DMC U/ml (NP),
 15-20 min, 37 °C
0.3 PZ U/ml (NB 4), 
30-45 min, 37 °C

Protocol


Clinical Applications*

Species    

Enzyme

Conditions

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Human*

Collagenase NB 6

0.3-0.4 PZ U/ml,
4-16 h, 37 °C

Protocol


*For further information on GMP grade Collagenase NB please contact us!

Collagenase NB 4 Standard Grade (cat. no. S1745401) has a collagenase activity of 
≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.

Collagenase NB 6 GMP Grade (cat. no. N0002779)  has a collagenase activity of 
≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Neutral Protease NB (cat. no. S3030111) is a protease from C. histolyticum. It has a proteolytic activity of ≥ 0.50 DMC U/mg.

Isolation conditions
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results. For further information on optimization of tissue digestion please visit our support section!

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