Isolation of neurons with Collagenase NB

In the following, application details for isolation of neurons from human and rodent dorsal root ganglia are presented.

Species  Enzymes  Conditions  Download
 Human*  Collagenase NB 4
Collagenase NB 6

0.15-0.25 PZ U/ml, 30-60 min, 37 °C

 Rodent Collagenase NB 4 0.15-0.25 PZ U/ml, 
30-60 h, 37 °C**

* For information on GMP grade Collagenase NB please contact us!
**4-5 consecutive digestion steps are recommended


Collagenase NB 4 Standard Grade (cat. no. S1745401) has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. 

Collagenase NB 6 GMP Grade (cat. no. N0002779 and N0002880) has a collagenase ac vity of ≥ 0.1 PZ U/mg and a balanced mix of proteoly c side ac vi es. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Isolation conditions
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results. 
For further information on optimization of tissue digestion please visit our support section!

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