Isolation of melanoma cells with Collagenase NB
In the following, application details for isolation of melanoma cells from murine skin are presented.
|Mouse||Collagenase NB 8||0.12 PZ U/ml, |
30 min, 37 °C
Collagenase NB 8 Broad Range (cat. no. S1745601) is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
For further information on optimization of tissue digestion please visit our support section!