Isolation of mastocytes with Collagenase NB

In the following, application details for isolation of mastocytes from human intestinal mucosa are presented.

Species Enzyme Conditions Download
Human* Collagenase NB 4G 0.5 PZ U/ml,
1 h, 37 °C

 * For information on GMP grade Collagenase NB please contact us!

Collagenase NB 4G Proved Grade (cat. no. S1746501) has a collagenase activity of ≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.

Isolation conditions
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results. 
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