Isolation of lung cells with Collagenase NB
In the following, application details for isolation of cells from murine lung are presented.
|Mouse||Collagenase NB 4|| 0.2 PZ U/ml, |
1 h, 37 °C
Collagenase NB 4 Standard Grade (cat. no. S1745401) has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
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