Lung Cells

Isolation of lung cells with Collagenase NB

In the following, application details for isolation of cells from murine lung are presented.

Species Enzyme Conditions Download
 Mouse Collagenase NB 4  0.2 PZ U/ml,
1 h, 37 °C
 Protocol

 

Collagenase NB 4 Standard Grade (cat. no. S1745401)  has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.

Isolation conditions
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results. 
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