Collagenase NB 8 Broad Range from Clostridium histolyticum
Nordmark Collagenase NB - first choice for high yield and viable cells
Suitable for a broad range of different applications in tissue dissociation such as isolation of rodent pancreatic islets of Langerhans, human cardiomyocytes, tumor cells, muscle cells, osteoblasts and porcine hepatocytes. Increased collagenase activity compared to Collagenase NB 4 Standard Grade (Cat. no. S1745401) and Collagenase NB 4G Proved Grade (Cat. no. S1746501).
Contains class I and class II collagenase (1) as well as reduced levels of clostripain, trypsin-like activities and neutral protease.
PZ activity (Wünsch): ≥ 0.90 U/mg
Endotoxin: ≤ 100 EU/mg (Ph. Eur.)
EC 22.214.171.124 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1]
|Collagenase NB 8 Broad Range||S1745601|| |
Collagenase NB 8 Broad Range
|S1745602||1 g|| |
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Storage Temperature: +2 °C to +8 °C
This article will be delivered cooled on blue ice.
If you are dealing with poor cell yields, collagenases with higher side activities like Collagenase NB 4 Standard Grade (Cat. No. S1745401) and Collagenase NB 4G Proved Grade (Cat. Co. S1746502).
References and Definitions
Collagenase: 1 U according to Wünsch (2) catalyzes the hydrolysis of
1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.
Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).
1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51.
H315 - H317 - H319 - H334 - H335
P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362
EINECS: 232-582-9 • WGK: 1 • HS: 35079090