Collagenase NB 7D Gentle Grade from Clostridium histolyticum

Nordmark Collagenase NB - first choice for high yield and viable cells

Collagenase NB 7D Gentle Grade is suitable for isolation of a broad variety of cells. Moreover, it can be used especially where sensitive conditions are favored as during isolation of dendritic and tumor cells from human or animal tissues. To meet these requirements, Collagenase NB 7D Gentle Grade combines the benefits of slightly higher collagenase activity with low tryptic activities ideal to preserve cell-surface proteins.

Application:

Collagenase NB 7D Gentle Grade can be used for a variety of different applications when sensitive cells are isolated.

More about Applications

Specifications:

Contains higher amounts of class I and class II collagenase (1) and lower trypsin-like activities.
PZ activity (Wünsch): ≥ 0.2 U/mg
(Clostridiopeptidase A)

Trypsin-like activities BAEE): ≤ 0.2 U/mg

Ratio Collagenase : Trypsin: ≥ 3.0

EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1]

Order Information

ProductnameCat.NoSizeEUR
Collagenase NB 7D Gentle GradeS1747401

500 mg

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Collagenase NB 7D Gentle GradeS17474021 g

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Collagenase NB 7D Gentle GradeS17474035 gInquire+

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Storage Temperature: +2 °C to +8 °C

Related Products

If you are dealing with less efficient cell isolation results, a product with higher tryptic activities like, Collagenase NB 4G Proved Grade (Cat. No. S1746502) might be better suitable. However, in case of poor cell viability, try a product with even less tryptic activities like Collagenase NB 1 Premium Grade (Cat. No. S1745503).

References and Definitions

Unit definition: 
Collagenase: 1 U according to Wünsch (2) catalyzes the hydrolysis of
1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.
Trypsin-like activities (BAEE assay): 1 U catalyzes the cleavage of 1 µmol BAEE per minute at 25 °C, pH 8.0. Enzyme dissolved in 1 mM HCl.

References:
1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51.

Safety Information

Signal word

DANGER

Hazard Statements

H315 - H317 - H319 - H334 - H335

Precautions

P280

Reaction

P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 

EINECS: 232-582-9 • WGK: 1 • HS: 35079090

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