Collagenase NB 7D Gentle Grade can be used for a variety of different applications when sensitive cells are isolated.

More about Applications

Contains higher amounts of class I and class II collagenase (1) and lower trypsin-like activities.
PZ activity (Wünsch): ≥ 0.2 U/mg
(Clostridiopeptidase A)

Trypsin-like activities BAEE): ≤ 0.2 U/mg

Ratio Collagenase : Trypsin: ≥ 3.0

EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1]

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Storage Temperature: +2 °C to +8 °C

If you are dealing with less efficient cell isolation results, a product with higher tryptic activities like, Collagenase NB 4G Proved Grade (Cat. No. S1746502) might be better suitable. However, in case of poor cell viability, try a product with even less tryptic activities like Collagenase NB 1 Premium Grade (Cat. No. S1745503).

Unit definition: 
Collagenase: 1 U according to Wünsch (2) catalyzes the hydrolysis of
1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.
Trypsin-like activities (BAEE assay): 1 U catalyzes the cleavage of 1 µmol BAEE per minute at 25 °C, pH 8.0. Enzyme dissolved in 1 mM HCl.

References:
1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51.

Deutsch:

Sicherheitsdatenblatt

English:
Product Information
Material Safety Datasheet
Collagenase NB 7D Flyer
CoO (Certificate of Origin)
Certificate of Analysis (Lotsearch)

EINECS: 232-582-9 • WGK: 1 • HS: 35079090