World's first animal free GMP Grade Collagenase
Collagenase AF-1 GMP Grade is derived from C. histolyticum using a production process completely free of any animal based components. Thus, any risk of transmission of potential animal-derived pathogens is excluded. This highly purified collagenase, often in combination with Neutral Protease AF GMP Grade (Cat. No. N0002936), is suitable for the dissociation of sensitive cells from several tissues, such as pancreas, liver and tumor. It has been shown to be highly effective for the isolation of islets of Langerhans from human pancreas intended for transplantation and stromal vascular fraction (SVF) from adipose tissue and tumor cells.
Collagenase AF-1 GMP can be used for several cell isolations. Our bigger packaging size of ≥ 2000 PZ U is ideal for human islet isolation, whereas the samller size of ≥ 150 PZ U can be used for tumor cell isolation, isolation of TILs or SVF from adipose tissue. More about Applications
PZ activity (Wünsch) ≥ 3.00 U/mg*.
Endotoxin ≤ 10.0 EU**/mg (Ph. Eur.)
Microbiological examination: Total aerobic microbial count (TAMC), total combined yeast/moulds count (TYMC), absence of Clostridia.
Endproduct is 0.22 µm filtered.
Manufactured according to EU GMP guidelines.
(Clostridiopeptidase A, Collagenase A)
EC 220.127.116.11 • Mr Mr 70 000 - 120 000 (collagenase) • CAS [9001-12-1]
|Collagenase AF-1 GMP Grade||N0003554||≥ 2,000 PZ U*||Inquire+|
|Collagenase AF-1 GMP Grade||N0003855||≥ 150 PZ U*||Inquire+|
+Please use the inquiry form in the right column.
Storage Temperature: +2 °C to +8 °C
This article will be delivered cooled on blue ice.
Collagenase AF-1 GMP Grade is often used in combination with Neutral Protease AF GMP Grade (Cat. No. N0003553). For research purpose, Nordmark provides research grade alternatives like Collagenase NB 1 Premium Grade (Cat. No. S1745503) and Neutral Protease NB (Cat. No. S3030112).
*Unit definitions:Collagenase: 1 U according to Wünsch (2) catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.
**Endotoxin: Ph. Eur. [1 Endotoxin Unit is equal to 1 International Unit of a WHO appoved reference standard endotoxin (RSE)].
1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler´s Z. Physiol. Chem. 333, 149-51
H315 - H317 - H319 - H334 - H335
P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311 - P362 +P364