Neutral Protease AF GMP Grade from Clostridium histolyticum

World's first animal free GMP Grade Neutral Protease

Neutral Protease AF GMP Grade is derived from C. histolyticum using a production process completely free of any animal based components. Thus, any risk of transmission of potential animal-derived pathogens is excluded. This purified metalloprotease is suitable for the dissociation of tissue in clinical applications. For especially gentle cell isolation it is recommended to use Neutral Protease AF GMP Grade in combination with highly purified Collagenase AF-1 GMP Grade (Cat. No. N0003554).

Application:

More about Applications

Specifications:

Plant-based fermentation process. Contains chromatographically purified neutral protease.
Vial contains not less than 100 DMC U of neutral protease.

Neutral protease activity (DMC) ≥ 0.50 U/mg.

Endotoxin: ≤ 100 EU/mg (Ph. Eur.)

Largely free of collagenolytic activity.

Microbiological examination: Total aerobic microbial count (TAMC), total combined yeast/moulds count (TYMC), absence of Clostridia
Endproduct is 0.22 µm filtered.

Manufactured according to EU GMP guidelines.

M_r M_r approx. 34 000 • CAS [9001-92-7]

Order Information

Productname	Cat.No	Size	EUR
Neutral Protease AF GMP Grade	N0003553	≥ 100 DMC-U	Inquire⁺

⁺Please use the inquiry form in the right column.

Storage Temperature: +2 °C to +8 °C
This article will be delivered cooled on blue ice.

Related Products

Neutral Protease AF GMP Grade is often used in combination with Collagenase AF-1 GMP Grade (Cat. No. N0003554). If your application is in the field of research, Nordmark provides research grade alternatives like Neutral Protease NB (Cat. No. S3030112) and Collagenase NB 1 Premium Grade (Cat. No. S1745503).

References and Definitions

Unit definition:
Neutral Protease: 1 DMC Unit catalyzes the cleavage of 1 µmol peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS (1).
Endotoxin: Ph. Eur. [1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)].

References:
1. Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93