Thyriod

Isolation of cells from thyriod with Collagenase NB

In the following, application details for isolation of epithelial cells from human thyroid glands and human thyroid tumor tissue are presented.

Epithelia cells from human thyroid glands:

Species

 Enzymes

 Conditions

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 Human*

Collagenase NB 4
or
Collagenase NB 6

0.25-0.3 PZ U/ml, 
RT

Protocol


Tumor cells from human thyroid glands:

Species

 Enzymes

 Conditions

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Human*

Collagenase NB 8

3.5-5.5 PZ U/ml,
3-5 h, 37 °C

Protocol


* For information on GMP grade Collagenase NB please contact us!

Collagenase NB 4 Standard Grade (cat. no. S1745402) has a collagenase activity of 
≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. 

Collagenase NB 6 GMP Grade (cat. no. N0002779)  has a collagenase activity of 
≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Collagenase NB 8 Broad Range (cat. no. S1745601) is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.

Isolation conditions
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results. For further information on optimization of tissue digestion please visit our support section!

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