Skin

Isolation of skin cells with Collagenase NB

In the following, application details for isolation of endothelial cells from rodent aortas, human foreskin (HDMEC) and for isolation of fibroblasts from rodent and human skin and connective tissue are presented

Endothelia cells from from rodent aortas and human foreskin:

Species

 Enzymes

 Conditions

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 Rodent

Collagenase NB 4

 0.12-0.15 PZ U/ml, 
10-20 min, 37 °C

Protocol

 Human*
 (HDMEC)

Collagenase NB 4
or
Collagenase NB 6

0.5-0.7 PZ U/ml, 
0.5-2 h, 37 °C

Protocol


Melanoma cells from murine skin:

Species

 Enzymes

 Conditions

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Mouse

Collagenase NB 8

0.12 PZ U/ml,
30 min, 37 °C

Protocol


Fibroblasts from murine and human skin:

Species

 Enzymes

 Conditions

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 Human*

 Collagenase NB 4G

 0.2-0.3 PZ U/ml,
2 h, 37 °C

Protocol

 Rodent

Collagenase NB 4G

0.2-0.5 PZ U/ml, 
12-20 h, 37 °C

Protocol


Clinical applications:

Species

Enzyme

Conditions

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Human*

Collagenase NB 6 

0.2-0.3 PZ U/ml,
2 h, 37 °C

Protocol


* For information on GMP grade Collagenase NB please contact us!

Collagenase NB 4 Standard Grade (cat. no. S1745402) has a collagenase activity of 
≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. 

Collagenase NB 4G Proved Grade (cat. no. S1746501) has a collagenase activity of 
≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.

Collagenase NB 6 GMP Grade (cat. no. N0002779)  has a collagenase activity of 
≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Collagenase NB 8 Broad Range (cat. no. S1745601) is characterized by a high  collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities. 

Isolation conditions
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results. For further information on optimization of tissue digestion please visit our support section!

Contact Nordmark Biochemicals Team


 

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