Reproductive

Isolation of cells of reproductive tissues with Collagenase NB

In the following, application details for isolation of  Xenopus Oocytes, isolation of cells from human endometrium, human foreskin (HDMEC) and umbilical cord (HUVEC), epithelial cells from murine mammary glands and breast or uterus tumor cells are presented.

Oocytes from Xenopus laevis:

Species

Enzyme

Conditions

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Frog

Collagenase NB 4G

0.3-0.5 PZ U/ml,
1.5-2 h, RT

Protocol


Amnion and chorion cells from human fetal tissue:

Species

Enzyme

Conditions

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Human*

Collagenase NB 8

3.5-5.5 PZ U/ml,
3-5 h, 37 °C

Protocol


Endometrial cells from human cervical tissue:

Species

Enzyme

Conditions

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Human*

Collagenase NB 4
or
Collagenase NB 6

0.2-0.3 PZ U/ml, 
30 min, 37 °C

Protocol


Endothelia cells from human foreskin and umbilical vein:

Species

Enzyme

Conditions

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Human*
 (HDMEC)

Collagenase NB 4
or
Collagenase NB 6

0.5-0.7 PZ U/ml, 
0.5-2 h, 37 °C

Protocol

 Human*
 (HUVEC)

Collagenase NB 4
or
Collagenase NB 6

0.15-0.25 PZ U/ml, 
0.5-2 h, 37 °C

Protocol


Epithelia cells from murine mammary gland:

Species

Enzyme

Conditions

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Mouse

Collagenase NB 4

 0.3-0.4 PZ U/ml, 
15-25 min, 37 °C

Protocol


Tumor cells from breast and uterus:

Species

Enzyme

Conditions

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Human*

Collagenase NB 8

3.5-5.5 PZ U/ml,
3-5 h, 37 °C

Protocol


 For information on GMP grade Collagenase NB please contact us!

Collagenase NB 4 Standard Grade (cat. no. S1745402) has a collagenase activity of 
≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. 

Collagenase NB 4G Proved Grade (cat. no. S1746501) has a collagenase activity of 
≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.

Collagenase NB 6 GMP Grade (cat. no. N0002779)  has a collagenase activity of 
≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Collagenase NB 8 Broad Range (cat. no. S1745601)  is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities. 

Isolation conditions
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results. 
For further information on optimization of tissue digestion please visit our support section!

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