Neural

Isolation of neural cells with Collagenase NB

In the following, application details for isolation of neurons from human and rodent dorsal root ganglia and Schwann cells from nerve tissue are presented

Species Enzymes Conditions Downloads
Human* Collagenase NB 4 or Collagenase NB 6 0.15-0.25 PZ U/ml, 30-60 min, 37 °C  
Rodent Collagenase NB 4 0.15-0.25 PZ U/ml, 30-60 h, 37 °C Protocol


* For information on GMP grade Collagenase NB please contact us!

Collagenase NB 4 Standard Grade (cat. no. S1745401) has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.

Collagenase NB 6 GMP Grade (cat. no. N0002779)  has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Schwann cells from neuronal tissue:

Species

Enzyme

Conditions

Download

Rodent

Collagenase NB 4

0.2-0.3 PZ U/ml,
1-3 h, 37 °C

Protocol


Clinical applications*

Species

Enzyme

Conditions

Download

Human

Collagenase NB 1 GMP Grade,
Neutral Protease NB GMP Grade

0.5 PZ U/ml, 2 DCM U/ml, 
18 h, 37 °C

Protocol


* For information on GMP grade Collagenase NB please contact us!

Collagenase NB 1 GMP Grade (cat. no. N0002937) is a highly purified collagenase with a high collagenolytic activity (≥ 3.0 PZ U/mg) and very low proteolytic side activities. Research quality Collagenase NB 1 Premium Grade (cat. no. S1745503) is also available.

Collagenase NB 4 Standard Grade (cat. no. S1745401) has a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.

Neutral Protease NB GMP Grade (cat. no. N0002936) is a protease from C. histolyticum. It has a proteolytic activity of ≥ 0.50 DMC U/mg. It is also available in research quality Neutral Protease NB (cat. no. S3030111).

Isolation conditions
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results. 
For further information on optimization of tissue digestion please visit our support section!

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