Gastric/Intestine

Isolation of cells from gastric/ intestine tissue with Collagenase NB

In the following, application details for isolation of epithelial cells from gastric mucosa and mastocytes from human intestinal mucosa are presented

Species

Enzyme

Conditions

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 Rodent

Collagenase NB 4G

0.1-0.2 PZ U/ml, 
30-60 min, 37 °C

Protocol

Human*

Collagenase NB 4G

0.5 PZ U/ml,
1 h, 37 °C

Protocol


* For further information on GMP grade Collagenase NB please contact us!

Collagenase NB 4G Proved Grade (cat. no. S1746501) has a collagenase activity of 
≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.

Isolation conditions
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results. 
For further information on optimization of tissue digestion please visit our support section!

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