Bone

Isolation of osteoblasts with Collagenase NB

In the following, application details for isolation of osteoblasts from murine and human bone tissue are presented

Species

 Enzymes

 Conditions

 Download

 Human*

Collagenase NB 8,
Neutral Protease NB

1.5-2 PZ U/ml (NB 8), 
0.25 DMC U/ml (NP),
2-4 h , 37 °C 

Protocol

 Mouse

Collagenase NB 4

0.15-0.25 PZ U/ml, 
5-10 min, 37 °C**

Protocol

* For information on GMP grade Collagenase NB please contact us!

Collagenase NB 4 Standard Grade (cat. no. S1745401) shows a collagenase activity of ≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. 

Collagenase NB 8 Broad Range (cat. no. S1745601)  is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.

Neutral Protease NB (cat. no. S3030111) is a protease from C. histolyticum. It has a proteolytic activity of ≥ 0.50 DMC U/mg. 

Isolation conditions
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results. 
For further information on optimization of tissue digestion please visit our support section!

Contact Nordmark Biochemicals Team 

Product Inquiry

Product Inquiry

Product Inquiry Sidebar

Personal Consulting

E-Mail

Phone:
+49 (0)4122 / 712 560