Adipose/Fat

Isolation of cells from adipose/fat tissue with Collagenase NB

In the following, application details for isolation of adipocytes and ADSC from human or murine adipose tissue are presented.

Species

 Enzyme

 Conditions

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 Human

Collagenase NB 4

0.2-0.3 PZ U/ml*,
30-60 min, 37 °C

Protocol

 Murine

Collagenase NB 4

0.2-0.3 PZ U/ml**, 
30-60 min, 37 °C

Protocol


Clinical Applications**

Species

 Enzyme

 Conditions

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 Human

Collagenase NB 6

0.2-0.3 PZ U/ml*,
30-60 min, 37 °C

Protocol

*0.1-0.15 PZ U/ml final concentration after addition of adipose tissue
**For further information on GMP grade Collagenase NB please contact us!

Collagenase NB 4 Standard Grade (cat. no. S1745401) has a collagenase activity of 
≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.

Collagenase NB 6 GMP Grade (cat. no. N0002779) has a collagenase activity of 
≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Isolation conditions

The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
For further information on optimization of tissue digestion please visit our support section! 

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