Stem Cells

Isolation of stem cells with Collagenase NB

In the following, application details for isolation of stem cells from human Wharton’s jelly and human or murine adipose tissue are presented.

Adipose tissue:

Species  Enzyme  Conditions  Download
 Human Collagenase NB 4

0.2-0.3 PZ U/ml*,
30-60 min, 37 °C

Protocol
 Murine Collagenase NB 4 0.2-0.3 PZ U/ml*, 
30-60 min, 37 °C
Protocol

Clinical applications**:

Species Enzyme Conditions Download
Human Collagenase NB 6 0.2-0.3 PZ U/ml*,
30-60 min , 37 °C
Protocol

* 0.1 - 0.15 PZ U/ml final concentration after addition of adipose tissue

Wharton's jelly:

Species Enzyme Conditions Download
Human Collagenase NB 4 0.13-0.15 PZ U/ml,
1-6 h , 37 °C
Protocol

Clinical applications**:

Species Enzyme Conditions Download
Human Collagenase NB 6 0.13-0.15 PZ U/ml,
1-6 h , 37 °C
Protocol

**For further information on GMP grade Collagenase NB please contuct us!

Collagenase NB 4 Standard Grade (cat. no. S1745401) has a collagenase activity of 
≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. 

Collagenase NB 6 GMP Grade (cat. no. N0002779)  has a collagenase activity of 
≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia..

Isolation conditions
The suggested SERVA Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results. 
For further information on optimization of tissue digestion please visit our support section!

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