Schwann Cells

Isolation of Schwann cells with Collagenase NB

In the following, application details for isolation of Schwann cells from nerve tissue are presented.

Species Enzyme Conditions Download
Human Collagenase NB 1 GMP Grade &
Neutral Protease NB GMP Grade
0.5 PZ U/ml, 2 DCM U/ml, 
18 h, 37 °C
Protocol
Rodent Collagenase NB 4 0.2-0.3 PZ U/ml,
1-3 h, 37 °C
Protocol

 

Collagenase NB 1 GMP Grade (cat. no. N0002937) is a highly purified collagenase with a high collagenolytic activity (≥ 3.0 PZ U/mg) and very low proteolytic side activities. Research quality Collagenase NB 1 Premium Grade (cat. no. S1745503) is also available.

Neutral Protease NB GMP Grade (cat. no. N0002936) is a protease from C. histolyticum. It has a proteolytic activity of ≥ 0.50 DMC U/mg. It is also available in research quality Neutral Protease NB (cat. no. S3030111).

Collagenase NB 4 Standard Grade (cat. no. S1745401) has a collagenase activity of 
≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.

Isolation conditions
The suggested SERVA Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results. 
For further information on optimization of tissue digestion please visit our support section!

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