Oocytes

Isolation of oocytes with Collagenase NB

In the following, application details for isolation of oocytes from ovaries of frogs (Xenopus) are presented.

Species Enzyme Conditions Download
Frog Collagenase NB 4G 0.3-0.5 PZ U/ml,
1.5-2 h, RT
Protocol

Collagenase NB 4G Proved Grade (cat. no. S1746501) has a collagenase activity of 
≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.

Isolation conditions
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results. 
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