Isolation of oocytes with Collagenase NB
In the following, application details for isolation of oocytes from ovaries of frogs (Xenopus) are presented.
|Frog||Collagenase NB 4G||0.3-0.5 PZ U/ml, |
1.5-2 h, RT
Collagenase NB 4G Proved Grade (cat. no. S1746501) has a collagenase activity of
≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results.
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