Melanoma Cells

Isolation of melanoma cells with Collagenase NB

In the following, application details for isolation of melanoma cells from murine skin are presented.

Species Enzyme Conditions Download
Mouse Collagenase NB 8 0.12 PZ U/ml,
30 min, 37 °C
Protocol

 

Collagenase NB 8 Broad Range (cat. no. S1745601) is characterized by a high  collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities. 

Isolation conditions
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results. 
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