Islets of Langerhans

Isolation of islets of Langerhans with Collagenase NB

In the following, application details for isolation of islets of Langerhans from human, rodent and porcine pancreatic tissue are presented.

Species Enzymes Conditions Download
Human* Collagenase NB 1
Premium Grade,
Neutral Protease NB

Per gram tissue:
20 PZ U (NB 1),
0.8-1.5 DMC U (NP),
15-30 min, 37 °C

 
Porcine
(adult)
Collagenase NB 8,
Neutral Protease NB

Per gram tissue:
4.4 PZ U (NB 8),
0.5-0.7 DMC U (NP),
37 °C

Protocol 
Porcine
(neonatal)
Collagenase NB 4

0.17-0.2 PZ U/ml,
10-20 min, 37 °C

Protocol
Rodent Collagenase NB 8 1.5-1.7 PZ U/ml
14-17 min, 37 °C
Protocol 

Clinical Applications*

Species Enzymes Conditions Download
Human Collagenase NB 1
GMP Grade,
Neutral Protease NB
GMP Grade

Per gram tissue:
20 PZ U (NB 1),
0.8-1.5 DMC U (NP),
15-30 min, 37 °C

 
Porcine
(adult)
Collagenase NB 1
GMP Grade,
Neutral Protease NB
GMP Grade

Per gram tissue:
15-18  PZ U (NB 1),
0.8-1.3 DMC U (NP),
15-30 min, 37 °C

 
Porcine
(neonatal)
Collagenase NB 6

0.17-0.2 PZ U/ml,
10-20 min, 37 °C

 

 * For information on GMP grade Collagenase NB please contact us!

Collagenase NB 1 Premium Grade (cat. no. S1745504)  s a highly purified collagenase with a high collagenolytic activity (≥ 3.0 PZ U/mg) and very low proteolytic side activities. It is also available as GMP conformant Collagenase NB 1 GMP Grade (cat. no. N0002937).

Neutral Protease NB (cat. no. S3030111) is a protease from C. histolyticum. It has a proteolytic activity of ≥ 0.50 DMC U/mg. It is also available as GMP conformant Neutral Protease NB GMP Grade (cat. no. N0002936). 

Collagenase NB 4 Standard Grade (cat. no. SD1745401)  has a collagenase activity of 
≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities.

Collagenase NB 6 GMP Grade (cat. no. N0002779) has a collagenase activity of 
≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Collagenase NB 8 Broad Range (cat. no. S1745601) is characterized by ahight collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities.

Isolation conditions
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results. For further information on optimization of tissue digestion please visit our support section!

Contact Nordmark Biochemicals Team 

Product Inquiry

Product Inquiry

Product Inquiry Sidebar

Personal Consulting

E-Mail

Phone:
+49 (0)4122 / 712 560