Hepatocytes

Isolation of hepatocytes from liver tissue with Collagenase NB

In the following, application details for isolation of hepatocytes from rodent, porcine and human liver tissues are presented.

Species  Enzymes  Conditions  Download
Human*  Collagenase NB 4G 0.25-0.35 PZ U/ml,
10-20 min, 37 °C
Protocol
 Mouse  Collagenase NB 4G 0.08-0.11 PZ U/ml, 
5-15min, 37 °C
Protocol
 Pig   Collagenase NB 8 0.2-0.3 PZ U/ml Protocol
 Rat (adult)  Collagenase NB 4G  0.12-0.15 PZ U/ml, 
10-25 min, 37 °C
Protocol

Clinical applications*:

Species       Enzyme    Conditions Download
Human* Collagenase NB 6 0.25-0.35 PZ U/ml,
10-20 min, 37 °C
Protocol

 * For information on GMP grade Collagenase NB please contact us! 

Collagenase NB 4G Proved Grade (cat. no. S1746501) has a collagenase activity of 
≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.

Collagenase NB 8 Broad Range (cat. no. S1745601)  is characterized by a high collagenase activity (≥ 0.9 PZ U/mg) and reduced levels of proteolytic side activities. 

Collagenase NB 6 GMP Grade (cat. no. N0002779)  has a collagenase activity of 
≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Isolation conditions
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results. For further information on optimization of tissue digestion please visit our support section!

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