Isolation of fibroblasts with Collagenase NB

In the following, application details for isolation of fibroblasts from rodent and human skin and connective tissue are presented.

Species  Enzymes  Conditions  Download
 Human*  Collagenase NB 4G

 0.2-0.3 PZ U/ml,
2 h, 37 °C

 Rodent Collagenase NB 4G 0.2-0.5 PZ U/ml, 
12-20 h, 37 °C

Clinical applications:

Species Enzyme Conditions Download
Human* Collagenase NB 6  0.2-0.3 PZ U/ml,
2 h, 37 °C

 * For information on GMP grade Collagenase NB please contact us! 

Collagenase NB 4G Proved Grade (cat. no. S1746501) has a collagenase activity of 
≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.

Collagenase NB 6 GMP Grade (cat. no. N0002779)  has a collagenase activity of 
≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.

Isolation conditions
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results. For further information on optimization of tissue digestion please visit our support section!

Contact Nordmark Biochemicals Team 

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