Isolation of fibroblasts with Collagenase NB
In the following, application details for isolation of fibroblasts from rodent and human skin and connective tissue are presented.
|Human*||Collagenase NB 4G|| |
0.2-0.3 PZ U/ml,
|Rodent||Collagenase NB 4G||0.2-0.5 PZ U/ml, |
12-20 h, 37 °C
|Human*||Collagenase NB 6||0.2-0.3 PZ U/ml, |
2 h, 37 °C
* For information on GMP grade Collagenase NB please contact us!
Collagenase NB 4G Proved Grade (cat. no. S1746501) has a collagenase activity of
≥ 0.18 PZ U/mg and a balanced mix of proteolytic side activities.
Collagenase NB 6 GMP Grade (cat. no. N0002779) has a collagenase activity of
≥ 0.1 PZ U/mg and a balanced mix of proteolytic side activities. It is manufactured according to GMP guidelines and sterile according to European Pharmacopoeia.
The suggested Nordmark Collagenase NB concentrations and isolation conditions should be regarded as starting points. Collagenase NB dosage is recommended in PZ units to ensure consistent results. For further information on optimization of tissue digestion please visit our support section!